ABSTRACT
The objective of this study was to determine the ecological relationships between bacterial species that colonize infected root canals. Root canal bacteria recovered from one patient with pulp canal necrosis were evaluated in vitro for synergistic and antagonistic activities determined by mono and co-culture growth kinetics and the production of bacteriocin-like substances using the double layer diffusion method. Peptostreptococcus prevotii triggered a significant increase of Fusobacterium nucleatum growth, while the former bacteria did not affect the growth of P. prevotii. The bacterial species did not produce antagonism activity against itself or against any of the other two species. Despite many studies have demonstrated the capability of root canal microorganisms to produce antagonistic substances, these in vitro experimental tests show the synergistic effect of P. prevotii on the growth of F. nucleatum.
Subject(s)
Humans , Dental Pulp Cavity/growth & development , Dental Pulp Cavity/microbiology , Endodontics , Fusobacterium Infections , Fusobacterium nucleatum/growth & development , Gram-Positive Bacterial Infections , In Vitro Techniques , Peptostreptococcus/growth & development , Periapical Tissue/growth & development , Periapical Tissue/microbiology , Drug Synergism , Methods , Microbiology , MethodsABSTRACT
In endodontics, treatment of an open apex tooth with necrotic pulp is a problem. It seems that with promotion of remnants of Hertwig's epithelial sheath or rest of malassez accompany with a good irrigation of root canal we can expect root formation